#acl +All:read Default #format wiki #language en ---- Additional information can be found on the [[/Discussion|Discussion]] page. ---- '''Target Outcome''' Acquisition of cadaver knee specimens of desired types '''Target Specimens''' * ''Knee from a young, healthy, male donor.'' Donor age < 35 years. * ''Knee from a young, healthy, female donor.'' Donor age < 35 years. * ''Knee from an elderly, healthy, male donor.'' Donor age > 65 years. * ''Knee from an elderly, healthy, female donor.'' Donor age > 65 years. * ''Knee from an elderly, osteoarthritic, male donor.'' Donor age > 65 years; moderate based on Kellgren-Lawrence grading (1,2). * ''Knee from an elderly, osteoarthritic, female donor.'' Donor age > 65 years; moderate based on Kellgren-Lawrence grading (1,2). Specimens should meet the following criteria: * Entire leg from hip to ankle. * Age: 18-35 years old OR 65-80 years old * Height: 1.50 m - 1.90 m * Weight: 45 kg - 90 kg * BMI: 18.5 - 24.9 (normal range) * Serologically tested. Specimens will be excluded when * Knee injury, surgeries, or inflammatory arthritis. '''Secondary Specimens''' Besides the primary specimens described above, more specimens can be tested in future to include conditions such as obesity, and different gender and age combinations. These will be listed in this section. '''Specimen Acquisition''' * Science Care Inc. (Phoenix, AZ), http://www.sciencecare.com/ * National Disease Research Interchange (Philadelphia, PA), http://ndriresource.org/ * Anatomy Gifts Registry (Hanover,MD), http://www.anatomygifts.org/ '''Deliveries should be to:''' * Snehal Chokhandre Lerner Research Institute, 2111 E. 96th St. Cleveland, OH. 44195 '''Specimen Evaluation''' * Radiological data (frontal plane x-rays with the joint under axial loading) will be obtained prior to ordering the specimen to assess existence and severity of osteoarthritis. * Before testing, the specimens will be evaluated by an orthopaedic surgeon for their suitability to their assigned group. When possible, pre-purchased radiographic information will be utilized for this purpose. * During specimen preparation (see [[Specifications/SpecimenPreparation]]), the specimens will be evaluated by an orthopaedic surgeon following joint level dissection. * After anatomical imaging (see [[Specifications/ExperimentationAnatomicalImaging]]), MRI of the specimens will be evaluated by an orthopaedic surgeon. * During tissue sampling (see below and also [[Specifications/ExperimentationTissueMechanics]]), the tissue structures of the specimens will be evaluated by an orthopaedic surgeon. '''Specimen Storage''' * Specimens will be delivered from the company frozen on dry ice and stored at -20 ˚C. * See the specimen preparation page for more details on [[Specifications/SpecimenPreparation]] '''Specimen Numbering Convention''' * Specimen will be numbered irrespective of their type as they are acquired and tested, e.g., 001, 002. For more details, refer to [[Specifications/DataManagement]]. '''Tissue Samples from Specimens''' * Primary samples: ACL, PCL, MCL, LCL, menisci, medial and lateral femoral and tibial cartilage, patellar cartilage, cartilage in femoral groove, capsule, patellar ligament, quadriceps tendon, MPFL. At least one sample from each tissue will be required for mechanical testing and following constitutive modeling. For more details, refer to [[Specifications/SpecimenPreparation]]. * Secondary samples: When possible, additional tissue samples will be prepared along with the primary samples and frozen for later use. This may include different tissue types or more sampling of the same tissue from different regions. For more details, refer to [[Specifications/SpecimenPreparation]]. * Tissue sample numbering convention will include the specimen number, tissue type (three letter abbreviation), location within tissue and sample number. For more details, refer to [[Specifications/DataManagement]]. '''References''' 1. Berger MJ, Kean CO, Goela A, Doherty TJ. ''Disease severity and knee extensor force in knee osteoarthritis: Data from the Osteoarthritis Initiative.'' Arthritis Care Res (Hoboken). 2012 May;64(5):729-34. http://www.ncbi.nlm.nih.gov/pubmed/22238225 1. Braun HJ, Gold GE. ''Diagnosis of osteoarthritis: imaging.'' Bone. 2012 Aug;51(2):278-88. http://www.ncbi.nlm.nih.gov/pubmed/22155587