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This page provides specifications of knee specimen '''OKS008'''. This is a middle age (likely healthy) male specimen candidate.
<<TableOfContents>>
== Specimen Characteristics ==
Right knee
'''Gender:''' ''Male''
'''Age:''' ''40 years''
'''Race:''' ''White ''
'''Height:''' ''1.778 m''
'''Weight:''' '' 63.5 kg''
'''BMI:''' ''20.09 ''
'''Serologically tested:''' ''Yes.''
''' X-ray: '''
[[attachment:oks008.png|{{attachment:oks008.png|oks008 x-ray|width=300}}]]
== Experimentation ==
-- [[aerdemir]] <<DateTime(2015-06-01T14:35:32Z)>> All, please complete relevant sections of this page during and after experimentation.
=== Specimen ===
'''Reference Specification:''' [[Specifications/Specimens]] - [[http://wiki.simtk.org/openknee/Specifications/Specimens?action=recall&rev=40|Revision as of June 1, 2015]]
'''Leading Team Member:''' Snehal Chokhandre
'''Supporting Team Members:''' Ahmet Erdemir, Omar Gad
'''Timeline:''' June 1, 2015 @ ~ 9:30 AM (specimen pulled out of freezer)
'''Data Location:'''
'''Protocol Deviations:'''
1. ''Specimen Characteristics.'' Donor age (40 yrs) does not match specifications.
'''Notes:'''
1. None.
=== Specimen Preparation ===
'''Reference Specification:''' [[Specifications/SpecimenPreparation]] - [[http://wiki.simtk.org/openknee/Specifications/SpecimenPreparation?action=recall&rev=141|Revision as of June 2, 2015]]
'''Leading Team Member:''' Tara Bonner
'''Supporting Team Members:''' Callan Gillespie, Snehal Chokhandre, Craig Bennetts
'''Timeline:'''
. Thawing: ~9:30 AM June 1, 2015 - ~9:30 AM June 2, 2015
. Dissection: ~9:30 AM - ~11:30 AM June 2, 2015
. Assembly of Optotrak Base Plugs: ~12:00 PM - ~12:35 PM June 2, 2015
. Assembly of Registration Markers: ~12:35 PM - ~1:30 PM June 2, 2015
. Probing of Anatomical Landmarks: ~2:00 PM - ~3:00 PM June 2, 2015
. Probing of Registration Markers: ~2:00 PM - ~3:00 PM June 2, 2015
'''Data Location:'''
'''Protocol Deviations:'''
1. None.
'''Notes:'''
1. Dissected and keeping skin-fat-muscle layers from thigh and shank.
1. The orientation of the bone plug for tibia was slightly tilted to accommodate motion analysis marker in relation to pot.
1. The femur registration markers were placed to medial, lateral, and posterior sides (alternative configuration).
1. The femur registration marker digitization sequence was medial, lateral, posterior.
1. Dissected and keeping the femoral head.
1. Dissected and keeping bone from femoral and tibial shafts.
=== Joint Imaging ===
==== Specimen Preparation ====
'''Reference Specification:''' [[Specifications/SpecimenPreparation]] - [[http://wiki.simtk.org/openknee/Specifications/SpecimenPreparation?action=recall&rev=141|Revision as of June 2, 2015]]
'''Leading Team Member:''' Craig Bennetts
'''Supporting Team Members:''' Snehal Chokhandre
'''Timeline:''' ~3:00 PM - ~4:00 PM June 2, 2015 (patella registration marker will be placed next day)
'''Data Location:'''
'''Protocol Deviations:'''
1. None.
'''Notes:'''
1. None.
==== Imaging ====
'''Reference Specification:''' [[Specifications/ExperimentationAnatomicalImaging]] - [[http://wiki.simtk.org/openknee/Specifications/ExperimentationAnatomicalImaging?action=recall&rev=121|Revision as of June 3, 2015]]
'''Leading Team Member:''' Craig Bennetts
'''Supporting Team Members:''' Snehal Chokhandre, Omar Gad, Ahmet Erdemir
'''Timeline:'''
. Specimen Placement in Transportation Fixture: ~8:30 AM - ~9:30 AM June 3, 2015
. Imaging: ~10:00 AM - ~12:30 PM June 3, 2015
'''Data Location:'''
Data was received from the imaging facilities and uploaded to in-house data management server (http://cobicore.lerner.ccf.org/midas; only accessible within Cleveland Clinic network). Raw DICOM files were stored in the folder Open Knee(s) --> Private --> oks008 --> MRI --> DICOM
DICOM files were converted to NIfTI files, stored in the folder Open Knee(s) --> Private --> oks008 --> MRI --> NIFTI of the in-house data management server. NIfTI files were also disseminated to the public in the 'Downloads' section of the project website, https://simtk.org/home/openknee, under the package oks008.
Data storage and dissemination were accomplished based on the updated imaging specifications, see [[http://wiki.simtk.org/openknee/Specifications/ExperimentationAnatomicalImaging?action=recall&rev=121|Revision as of June 26, 2015]].
'''Protocol Deviations:'''
1. None.
'''Notes:'''
1. Skin, fat and muscle layer from posterior region of the knee needed to be removed to fit into specimen transportation and imaging fixture.
1. General purpose imaging
[[attachment:oks008_general.png|{{attachment:oks008_general.png|oks008 General Purpose Imaging|width=600}}]]
1. Cartilage imaging
[[attachment:oks008_cartilage.png|{{attachment:oks008_cartilage.png|oks008 Cartilage Imaging|width=600}}]]
1. Connective-tissue imaging
[[attachment:oks008_connective.png|{{attachment:oks008_connective.png|oks008 Connective Tissue Imaging|width=600}}]]
=== Joint Mechanics ===
==== Equipment Preparation ====
'''Reference Specification:''' [[Specifications/PressureCalibration]] - [[http://wiki.simtk.org/openknee/Specifications/PressureCalibration?action=recall&rev=17|Revision as of June 3, 2015]]
'''Leading Team Member:''' Robb Colbrunn
'''Supporting Team Members:''' Nicholas Haas, Diana Suciu, Connor Lough
'''Timeline:''' ~9:00 AM - ~10:30 PM June 3, 2015
'''Data Location:'''
'''Protocol Deviations:'''
1. None.
'''Notes:'''
1. None.
==== Specimen Preparation ====
'''Reference Specification:''' [[Specifications/SpecimenPreparation]] - [[http://wiki.simtk.org/openknee/Specifications/SpecimenPreparation?action=recall&rev=141|Revision as of June 3, 2015]]
'''Leading Team Member:''' Tara Bonner
'''Supporting Team Members:''' Callan Gillespie
'''Timeline:'''
. Specimen preparation for TFJ testing: ~12:45 PM - ~2:00 PM June 3, 2015
. Specimen preparation for PFJ testing: ~7:30 AM - ~9:45 AM June 4, 2015
'''Data Location:'''
'''Protocol Deviations:'''
1. None.
'''Notes:'''
1. Drill bits to secure the pots were not placed during specimen preparation. This was noticed during the first reproducibility test of the tibiofemoral joint. The specimen was taken down from the robot for placement of drill bits.
==== Testing ====
'''Reference Specification:''' [[Specifications/ExperimentationJointMechanics]] - [[http://wiki.simtk.org/openknee/Specifications/ExperimentationJointMechanics?action=recall&rev=97|Revision as of June 3, 2015]]
'''Leading Team Member:''' Robb Colbrunn
'''Supporting Team Members:''' Callan Gillespie, Tara Bonner
'''Timeline:'''
. Specimen Mounting for TFJ testing: ~2:00 PM - ~2:30 PM June 3, 2015
. Testing of TFJ: ~2:30 PM -6:30 PM ~ June 3, 2015
. Specimen Mounting for PFJ testing: ~9:45 AM - June 4, 2015
. Testing of PFJ: June 4, 2015
'''Data Location:'''
Data was received from the robotics testing facility and uploaded to in-house data management server (http://cobicore.lerner.ccf.org/midas; only accessible within Cleveland Clinic network). Zipped folders were stored in the folder Open Knee(s) --> Private --> oks008 --> !JointMechanics under relevant joint related folders.
Zipped folders were extracted and organized in the dissemination folder under Open Knee(s) --> Private --> oks008 --> !JointMechanics --> DISSEMINATION of the in-house data management server. DISSEMINATION folder was downloaded and disseminated to the public in the 'Downloads' section of the project website, https://simtk.org/home/openknee, under the package oks008.
Data storage and dissemination were accomplished based on the updated joint mechanics testing specifications, see [[http://wiki.simtk.org/openknee/Specifications/ExperimentationJointMechanics?action=recall&rev=102|Revision as of October 21, 2015]].
'''Protocol Deviations:'''
1. None.
'''Notes:'''
1. Drill bits to secure the pots were not placed during specimen preparation. This was noticed during the first reproducibility test of the tibiofemoral joint. The specimen was taken down from the robot for placement of drill bits and remounted afterwards. The first reproducibility test (AP laxity at 30 degrees flexion) was repeated after placement of drill bits. The tibiofemoral joint experimentation continued as planned afterwards.
1. For tibiofemoral joint testing, at 0 degrees flexion testing conditions, the robot and the control computer lost communications. The system needed to be rebooted. The experiments continued starting with 0 degrees flexion testing.
1. For tibiofemoral joint testing, the final reproducibility test illustrated a shift in all translations (as observed from robot measured kinematics). The motion analysis measurements of tibiofemoral joint should be checked, if this is indeed the case.
1. Force data was filtered up through 200N 15 degrees PFJ Testing. After this point data was not filtered.
1. We will need to check and see if previous PFJ Testing had force torque filtering. All TFJ Testing did not have filtering.
=== Tissue Mechanics ===
==== Specimen Preparation ====
'''Reference Specification:'''
'''Leading Team Member:''' Morgan Jones, MD
'''Supporting Team Members:''' Snehal Chokhandre, Craig Bennetts, Omar Gad, Conner Lough, Diana Suciu.
'''Timeline:'''
1. Soft tissue dissection: ~2:15 PM - ~3:30 PM June 4, 2015
1. Cartilage dissection: ~10:30 AM - 1:00 PM June 5, 2015
'''Data Location:'''
'''Protocol Deviations:'''
1. Due to delay in patellofemoral joint testing, tissue dissection was not completed on the same day; all ligament, tendon, menisci segments were dissected and frozen. Bone and cartilage were kept in the fridge another night for dissection in the following morning.
'''Notes:'''
1. Secondary tissues dissected and saved: Transverse ligament
==== Testing ====
'''Reference Specification:'''
'''Leading Team Member:'''
'''Supporting Team Members:'''
'''Timeline:'''
'''Data Location:'''
'''Protocol Deviations:'''
1. None.
'''Notes:'''
1. None.
== Data Analysis ==
=== Registration ===
==== Spherical Markers ====
1. Femur
1. <Posterior>oks008_MRG_FMR-P_AGS_01.stl
1. <Lateral>oks008_MRG_FMR-L_AGS_01.stl
1. <Medial>oks008_MRG_FMR-M_AGS_01.stl
1. Tibia
1. <Posterior>oks008_MRG_TBR-P_AGS_01.stl
1. <Lateral>oks008_MRG_TBR-L_AGS_01.stl
1. <Medial>oks008_MRG_TBR-M_AGS_01.stl
1. Patella
1. <Superior>oks008_MRG_PTR-S_AGS_01.stl
1. <Lateral>oks008_MRG_PTR-L_AGS_01.stl
1. <Medial>oks008_MRG_PTR-M_AGS_01.stl
==== Digitizing Order ====
1. Tibia:
* Sphere 1 = Lateral
* Sphere 2 = Medial
* Sphere 3 = Posterior
1. Femur:
* Sphere 1 = Medial
* Sphere 2 = Lateral
* Sphere 3 = Posterior
=== Joint Mechanical Testing ===
Pictured below are the Anterior Posterior laxity graph, the IR/ER laxity graph, and the Varus/Valgus laxity graph.
{{attachment:laxitygraphs.008.png}}
== Modeling & Simulation ==