Thanks,Robert. I centered my AMBER trajectories and now I can continue with MSM analysis without this data range issue.
But I wonder why use int16 but not int32 or int64? I'm not familiar with programing,maybe for the purpose of saving RAM space?

I'm new with the MSMbuilder, while I followed the tutorial of alanine dipeptide without any problem, I encounter the problem of "Data range too large for int 16" with my own testing trajectory . My input file is a 20ns chymotrypsin(with approximately 3000 atoms for dry protein ) run using AMBER, whi...