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Respected sir , I have provided path actuator to the gait 2354 model with 43% actuation but I didn't understand the Y-axis values which are shown in the excitation editor (Graph) is given below.
I have calculated metabolic rate by providing 15 N optimal force but this metabolic rate hasn't decreased from 43% then how I can say that I have provided 43% actuation.
How 43% actuation works ?
43% actuation is depends on what parameters?
Opensim has been provided " Plantar flexion Assist Curves (13%,43%,54%)in Gait10dof18musc.folder.I have taken 43% actuation data and draw the graph but this is not match to the excitation editor @ 43% actuation.
Please Help.

how I can say that I have provided 43% actuation

Please see the following paper:
Malcolm P, et al. (2013) A simple exoskeleton that assists plantarflexion can reduce the metabolic cost of human walking. PLoS ONE 8(2): e56137. https://doi.org/10.1371/journal.pone.0056137
The 13p, 43p, and 54p files in your Resources\Models\Gait10dof18musc\PlantarFlexorAssistCurves directory refer to actuation profiles that begin at around 13%, 43%, and 54% of the gait cycle (as described in the paper)---not 13%, 43%, and 54% savings in metabolic cost. The following example in the documentation may also be useful: Simulation-Based Design to Reduce Metabolic Cost (https://simtk-confluence.stanford.edu/d ... bolic+Cost).

Respected sir,
I can't understand what is the relationship between these Y-axis values with 43% controller.
Please Help.

Please see the paper by Malcolm et al. (2013), linked above. Specifically, see Supporting Information, Figure S1, panel B, bright green line.