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I am an undergraduate research assistant (aerospace engineering major) aiding in biomedical research. We are trying to use OpenSim to study various subjects in a sitting position who extend their ankle forward against a force gauge until they fatigue for 5 minutes or so. The knee angle stays relatively at -90 degrees since it doesn't really change all that much. Is it possible to use OpenSim in this study if their is no real motion, or should I simulate isometric knee bending from -90 to - 89.5 degrees? We use iWorx to gather our data for accelerations, EMGs, and forces.

OpenSim is very flexible, so often what outputs you want from a simulation will inform how complex your simulation should be and what details to include. You can check out the Overview of OpenSim Workflows page on the documentation site to help guide you.