H36Q_012

10/02/05

Power = 10,000
Gain = 1.25V
Channel 1 y-axis
50 kHz - 3,000,000 points

open minitweezer file = data
ramp 7 3-20,100,0 on mt

artifact occasionally ?? - q transition = protein other is slightly higher force = regular artefact

good x,z,i/s
change to pH 5
~ 3 min at 20 pN Fx

ramp5_1 on laptop 3 - 10,100,0

a1	drift ?	
a2	high
a3	
a4	drift ?
a5	
a6	low
a7	high - drift ?
a8	
a9	
a10	
a11
a12	low   switched to file B 
a13	high
a14
a15
a16
a17
a18
a19	low
a20	mid

ramp5_2 	3-10,100,0

broke

ps1	free bead at position of experiments
ps2	free bead away from pipette tip
ps3	free bead after center trap and auto align

Stokes test = 2.9 um bead

Stiffness test at ~ 10 p.n. with beads nonspecifically stuck together done in x direction not y