These are the RNAstructure text interfaces for Windows for command line (DOS) use. Note that the thermodynamic parameter files and HMM files, *.dat and *.dh files are required for operation. You can either invoke a program from within these directories as current working directory or set the DATAPATH environment variable to indicate the directory of their location. An environment variable can be set on the command line with SET DATAPATH="C:\Documents and Settings\dhm\RNAstructure" or (on Windows XP) on the system properties dialog from the control panel under advanced and then clicking "Environment Variables." Note that setting them here requires a reboot. Most of these programs provide basic help if invoked with no parameters and more extensive help when invoked with the -h flag. Be sure to note the format of .seq files that are input to RNAstructure. There are several examples included here. These files require one or more comment lines that start with ";". Then there is a sequence title line and finally the sequence, terminated with "1". New in RNAstructure 5.01 is the ability to use FASTA sequence files. Note the required format as shown in bmorivector.fasta. Please report bugs to David Mathews: David_Mathews@urmc.rochester.edu