Data Arangement: Y ------------------ (X) A C G T ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (T) -0.7 -0.1 -0.7 -0.1 -------------------------------- STACKING ENERGIES : Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY TY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . . . . -1.0 . . . . . . . . . . . . . . -1.4 . . . . . . . . . . . . . . -1.3 . +0.7 . . . . . . . . . . . . -0.9 . +0.1 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY TY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . -1.5 . . . . . . . . . . . . . . -1.8 . . . . . . . . . . . . . . -2.2 . -0.5 . . . . . . . . . . . . -1.3 . -0.3 . . . . . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY TY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . -1.3 . . . . . . . +0.3 . . . . . . -2.2 . . . . . . . -0.6 . . . . . . -1.8 . +0.1 . . . . . -0.3 . +0.7 . . . . -1.4 . -0.6 . . . . . +0.1 . +1.2 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' TX TX TX TX AY CY GY TY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . -0.6 . . . . . . . +0.4 . . . . . . -1.3 . . . . . . . +0.1 . . . . . . -1.5 . +0.4 . . . . . -0.5 . +0.5 . . . . -1.0 . +0.3 . . . . . +0.7 . +0.7 . . . . .