To generate data for dot plots, first run Dynalign and generate a save file. Then run the dotplot utility, dynalign_dotplot. The command line parameters are: dynalign_dotplot input.dsv max_%_difference output.out where input.dsv is a dynalign savefile, max_%_difference is the maximum percent difference in free energy from the lowest free energy structure for which dots will be recorded (for example, use 20 for 20%). output.out is the name of the file that will be written with the dot plot data. The output format is: i j k l energy*20, with tab delimiters. i and j are the nucleotides paired in sequence 1, k and l are the nucleotides paired in sequence 2, and energy*10 is the lowest free energy structure that contains these pairs, where the free energy is in kcal/mol and the energy is multiplied by ten.