RNAstructure

Version 5.1

Copyright 1996 - 2010

by David H. Mathews


Available Modules for Prediction and Calculation

Break RNA Pseudoknots

This item, unique to RNA, breaks pseudoknots in an RNA secondary structure, "Break RNA Pseudoknots."


Dot Plot

This item allows the display of a dot plot using one of many save file types generated from various manipulations of a sequence. See "Dot Plot."


Draw

This item will draw any structure saved in the CT file format. See "Draw."


Dynalign

This item opens the Dynalign tool, which will find the sequence alignment and the lowest free energy common structure common to two sequences. See "Dynalign."


Efn2

This item will determine the free energy of a secondary structure saved in the CT file format. See "Efn2."


ProbKnot: Predict Structures Including Pseudoknots

This item will predict a maximum expected accuracy structure, including pseudoknots, from a partition function save file, and save the result as a CT file. See "ProbKnot."


Fold Single Strand

This item will open a folding window. This is the traditional structure prediction algorithm that finds lowest free energy structures. See "Fold."


Fold Bimolecular

This item will predict the intermolecular secondary structures of two oligonucleotides. See "Fold -> Bimolecular Folding."


Generate All Suboptimal Structures

This item allows the prediction of all possible secondary structures within a specified energy increment of the lowest free energy structure. See "Generate All Suboptimal Structures."


MaxExpct: Predict MEA Structure

This item predicts maximum expected accuracy structures, which are structures composed of the most probable pairs. See "Maximum Expected Accuracy."


OligoScreen

This item opens the OligoScreen tool, which determines thermodynamic parameters for a set of oligonucleotides. See "OligoScreen."


OligoWalk

This item opens the OligoWalk tool, which can help to determine an oligomer that will hybridize tightly to a target. See "OligoWalk."


Partition Function

This item uses a partition function calculation to predict base pair probabilities for any canonical pair in the sequence. See "Partition Function."


Partition Function Bimolecular

This item uses a partition function calculation to predict base pair probabilities for base pairs between two sequences. See "Partition Function -> Bimolecular Partitioning."


Refold

This item can be used to predict a different set of suboptimal secondary structures from a previously folded sequence or calculation. See "Refold."


Stochastic Sampling

This item generates a sample of secondary structures for a sequence, drawn from the complete folding ensemble. See "Stochastic Sampling."