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ProbablePair

ProbablePair creates a group of suboptimal structures that contain base pairs above a probability threshold.

USAGE: ProbablePair <pfs file> <ct file> [options]

Required parameters:

<pfs file> The name of a file containing base pair probabilities, generated by a partition function calculation.
<ct file> The name of a CT file to which output will be written.

Options which don't require added values:

-h, -H, --help Display the usage details message.

Options which require added values:

-t, -T, --threshold Specify the pairing threshold for pairs to be included.
This should be expressed as a number > 0.5 and <= 1.0.
Default is 0, which signifies structures should be generated at multiple thresholds: 0.99, 0.97, 0.95, 0.90, 0.80, 0.70, 0.60, and 0.50.

References

  1. Reuter, J.S. and Mathews, D.H.
    "RNAstructure: software for RNA secondary structure prediction and analysis."
    BMC Bioinformatics, 11:129. (2010).
  2. Mathews, D.H.
    "Using an RNA secondary structure partition function to determine confidence in base pairs predicted by free energy minimization."
    RNA, 10:1178-1190. (2004).