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bipartition

bipartition is used to predict the partition function for structures containing two strands. Note that the structures do not include those with intramolecular pairs.
Note that output is written to a partition function save file where the sequences are concatenated, with an intermolecular linker between them ("III").

USAGE: bipartition <seq file 1> <seq file 2> <pfs file> [options]

Required parameters:

<seq file 1> The name of a file containing a first input sequence. Note that lowercase nucleotides are forced single-stranded in structure prediction.
<seq file 2> The name of a file containing a second input sequence. Note that lowercase nucleotides are forced single-stranded in structure prediction.
<pfs file> The name of a partition function save file to which output will be written.

Options which don't require added values:

-d, -D, --DNA Specify that the sequence is DNA, and DNA parameters are to be used. Note that the backbone type must be the same for both sequences.
Default is to use RNA parameters.
-h, -H, --help Display the usage details message.

Options which require added values:

-t, -T, --temperature Specify the temperature at which calculation takes place in Kelvin.
Default is 310.15 K, which is 37 degrees C.

References:

  1. Reuter, J.S. and Mathews, D.H.
    "RNAstructure: software for RNA secondary structure prediction and analysis."
    BMC Bioinformatics, 11:129. (2010).
  2. Lu, Z.J. and Mathews, D.H.
    "Efficient siRNA Selection Using Hybridization Thermodynamics."
    Nucleic Acids Res., 36:640-647. (2007).