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refold

refold takes a previously folded sequence and can predict a new set of suboptimal structures.

USAGE: refold <save file> <ct file> [options]

Required parameters:

<save file> The name of a file containing folding information, generated by Fold.
This file must be a binary folding save file.
<ct file> The name of a CT file to which output will be written.

Options which don't require added values:

-h, -H, --help Display the usage details message.

Options which require added values:

-m, -M, --maximum Specify a maximum number of structures. Note that suboptimal structures are generated until either the maximum number of structures are reached or the maximum percent difference is reached (below).
Default is 20 structures.
-p, -P, --percent Specify a maximum percent difference in folding free energy change in suboptimal structures. Note that suboptimal structures are generated until either the maximum percent free energy difference is reached or until the maximum number of structures is reached (above).
Default is 10 percent (specified as 10, not 0.1).
-w, -W, --window Specify a window size.
Default is determined by the length of the sequence.

References

  1. Reuter, J.S. and Mathews, D.H.
    "RNAstructure: software for RNA secondary structure prediction and analysis."
    BMC Bioinformatics>/i>, 11:129. (2010).
  2. Mathews, D.H., Sabina, J., Zuker, M. and Turner, D.H.
    "Expanded sequence dependence of thermodynamic parameters provides improved prediction of RNA secondary structure."
    J. Mol. Biol., 288:911-940. (1999).