Engineering: - Document the code. - Document the high-level "flow". - Change DiseaseClass (or equivalent) to work for sources other than autworks. Science: - Get ranking for seeds in other sources. - Did the schizophrenia verification set include known seeds? - Does current algorithm optimize for sensitivity? If so, then consider optimizing for PPV instead. - Is it a good idea to intersect the different sources used in the forming the interaction network? - Or, explain why the current union of data sources for interaction network is a good idea - Think about setting the thresholds using a statistical method, eg, hypergeometric or gene set enrichment analysis (GSEA) - consider altering the structure of the interaction network as part of sensitivity analysis - add figure: show small network with counts and paths Writing: - Describe how Assaf's PPI_File was created. - Describe structure of the network (nodes, edges, number of connected components, average edges per node, distribution of number of edges per node, etc) - Describe what is already known about using hub genes. Future science: - Use other measures than betweenness centrality. - Consider using other sources for gene networks. - Count repeated genes within a pair? Should we consider the relatedness between two genes? - When searching through paths, should we retain only the shortest? Shortest + n? All? Other? - Can we validate algorithm for disease where gene network is likely very complete? - Is hold-out testing the best way to evaluate given that network for neuropsych genes is likely to be quite sparse? - Should we remove disease specific genes from the entire graph before computing betweenness centrality (to increase signal)? Known gene databases: Assaf's Reactome http://string-db.org/ http://tools.autworks.hms.harvard.edu/genes/20692/gene_sets https://gene.sfari.org/autdb/Welcome.do http://www.szgene.org/ http://bioinfo.mc.vanderbilt.edu/SZGR/ phenopedia: (http://64.29.163.162:8080/HuGENavigator/phenoPedia.do?firstQuery=Schizophrenia&cuiID=C0036341%20%20&typeSubmit=GO&check=y&which=2&pubOrderType=pubD) Interesting/Relevant websites: http://www.nature.com/nrg/journal/v12/n1/full/nrg2918.html http://www.ovarianresearch.com/content/pdf/1757-2215-6-92.pdf http://www.biomedcentral.com/1471-2105/7/219 http://nar.oxfordjournals.org/content/early/2014/07/24/nar.gku678.full http://www.biomedcentral.com/1752-0509/3/36/ <---- check this out http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063581/ <----- Also this http://www.biomedcentral.com/1752-0509/2/72 http://nar.oxfordjournals.org/content/37/suppl_2/W305.short http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0061505 http://bioinformatics.oxfordjournals.org/content/24/13/i277.long http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902235/ New gene? http://www.news-medical.net/news/20140704/Researchers-reveal-how-schizophrenia-linked-genetic-variation-alters-skeletons-of-developing-brain-cells.aspx