MBAR analysis of single-molecule DNA hairpin single-molecule equilibrium experiments under constant force load. DESCRIPTION REFERENCES [1] Woodside MT, Behnke-Parks WM, Larizadeh K, Travers K, Herschlag D, and Block SM. Nanomechanical measurements of the sequence-dependent folding landscapes of single nucleic acid hairpins. PNAS 103(16):6190-6195, 2006. [2] Greenleaf WJ, Woodside MT, Abbondanzieri EA, and Block SM. Passive all-optical force clamp for high-resolution laser trapping. PRL 95:208102, 2005. MANIFEST original-data/ - original data in Excel spreadsheet form, provided by Michael T. Woodside , corresponding author of [1] processed-data/ - external biasing forces (in pN) and extension trajectories (in nm, 0.1 ms time resolution) extracted from the Excel files in original-data/ extract-data.py - Python script to extract data from Excel datafiles (slow!) force-bias-optical-trap.py - Python script to compute potentials of mean force (PMFs) using MBAR plots/ - directory containing plots output/ - directory containing PMFs CORRESPONDENCE Correspondence with the corresponding author, Michael T. Woodside, describing the dataset appears below. --------------------------------------------------------------- Hi John, Here are 5s records, with data points every 0.1 ms, recorded at 16 different forces for the same molecule, hairpin 20R55/4T in the naming scheme I used in the paper. It's the same hairpin as for Fig. 1. I stuck the data in an Excel file. As far as errors go, there are some reasonably large systematic errors that can crop up in the force, so the absolute force values are known to within about 10% or so for a given molecule (bead size variations are a large factor here). As a first pass, by all means feel free to ignore the errors, but it's a good idea to keep them in mind for further down the road. Definitely, the file does not quote significant digits properly!! I have three more datasets that are ready to be sent, and several more to sort through. Hopefully this will give you something to work with for now... Cheers, --Michael --------------------------------------------------------------- Michael Woodside National Institute for Nanotechnology, NRC and Dept. of Physics, University of Alberta 11421 Saskatchewan Dr Edmonton AB, T6G 2M9 Canada tel: (780) 641-1695 fax: (780) 641-1601 ---------------------------------------------------------------