• RecurringMeetings
• 2017-09-05-Update

Biweekly Meeting of Cleveland Clinic Core Team

Date: Sept 05, 2017

Time: 10:30 AM EST

Means: In person meeting

Attendees:

1. Ahmet Erdemir
2. Snehal Chokhandre

Agenda:

1. Discuss ongoing action items from the last meeting.
2. Discuss progress of tissue testing protocol evaluation.
3. Discuss segmentation progress.
4. Decide ongoing action items for the next meeting.
5. Other.

Ongoing Action Items:

• Ahmet
  • Complete reviewing the drafts of data manuscripts.
• Snehal
  • Continue filling in the dashboard for tissue testing reproducibility.
• All
  • Continue segmentation based on commitments listed in the Roadmap.

Notes:

1. Discuss immediate action items from the last meeting.
   • Ahmet did not have any progress on reviewing the drafts of data manuscripts. Progress on tissue testing and segmentation were discussed as part of agenda items summarized below.
2. Discuss progress of tissue testing.
   • Snehal conducted a set of unconfined compression tests for meniscus, the repeatability was not acceptable. The sample was manually cut (5 mm by 5 mm rectangle). She will repeat another set.
   • Snehal also got a 3 mm thick rectangular filter made for confined compression tests.
   • Ahmet and Snehal decided that it is better to wrap up cartilage mechanical testing with a technical report as they have seen consistent acceptable repeatability for cartilage tests (including multiple tests over a week).
   • Snehal prepared a technical document which mainly focused on the mechanical tests in literature and evolution of the current testing protocols. Snehal and Ahmet discussed the document in detail. Ahmet suggested to add further details to the reference table. The protocol section needs to be subdivided into : tare load, preconditioning, and test. Other details needed are whether the tissue was hydrated and in what medium, the temperature at which the tests are conducted, pre-experiment stabilization, how was the sample thickness measured, detailed specimen preparation, how were the results calculated (moduli etc). Snehal will also add an illustration of the protocol (including the 300 micron buffer).
   • The document will also reference the studies that focused on effects of freezing on the cartilage.
   • Snehal will also conduct cartilage test with two weeks in between to assess the effects of long term storage in fridge on the properties. This is necessary because if 5 tests are conducted for each test type for each location the cartilage strip/sample will need to be kept in the fridge to avoid multiple freeze thaw cycles.
   • options for further studies were also discussed.
     • Using one location (e.g patella), prepare one compression and one tensile sample. Conduct unconfined compression, confined compression and tensile tests for 10 patella specimens.
     • Extend the study to multiple species.
     • Study the correspondence of indentation and extracted sample testing in cartilage tissue.
3. Discuss segmentation progress.
   • Snehal uploaded oks009 bones segmentation to the repository and Roadmap wiki page.