Biweekly Meeting of Cleveland Clinic Core Team
Date: Sept 25, 2017
Time: 10:30 AM EST
Means: In person meeting
- Ahmet Erdemir
- Snehal Chokhandre
- Discuss ongoing action items from the last meeting.
- Discuss progress of tissue testing protocol evaluation.
- Discuss segmentation progress.
- Decide ongoing action items for the next meeting.
Ongoing Action Items:
- Complete reviewing the drafts of data manuscripts.
- Continue filling in the dashboard for tissue testing reproducibility.
- Continue segmentation based on commitments listed in the Roadmap.
- Discuss immediate action items from the last meeting.
- Ahmet did not have any progress on reviewing the drafts of data manuscripts. Progress on tissue testing and segmentation were discussed as part of agenda items summarized below.
- Discuss progress of tissue testing.
- Snehal tested cartilage samples under unconfined compression, confined compression and tension in a week with 3 repeatability tests for each test type. The motivation was to be able to finish all tests for one location in a week with enough repeatability data. This was done to avoid keeping the sample in the fridge for too long (more than a week) and to avoid freezing the cartilage strip or sample. The unconfined compression repeatability appeared good. The forces seen in confined compression first test were lower than unconfined compression which indicated that their may be a problem with the set up. Snehal gently cleaned the outside of the filter with sand paper to reduce friction and the next two tests showed good repeatability. The compression tests were conducted over 3 day with unconfined compression test in the morning and after 2 hour wait confined compression test. Tensile tests were conducted on day 4 and 5. The repeatability appears acceptable for these two tests. The sample broke while taking out of the set up hence third test could not be conducted. The tensile sample was prepared at the beginning and kept in fridge during the compression tests.
- Snehal also conducted 2 cartilage unconfined compression tests with 2 weeks in between the tests. Sample was kept in the fridge between tests. The difference in the two peak forces and other parameters seems to be less than 10%. This test was done to assess the effect of long term storage of sample in fridge and to avoid multiple freeze thaw cycles if needed.
- Snehal also conducted 3 multi day tests for meniscus unconfined compression. The results are not acceptable. Snehal will look into this further.
- Snehal separated the literature table in cartilage mechanical testing technical report into specimen information and preparation and, testing specifications and results. She is also adding further references.
- Snehal and Ahmet discussed potential publications. For the first publication, it would be appropriate to focus on variability in procedures for mechanical characterization of cartilage in literature and development and standardization of testing protocol with documented repeatability. This will be done using one young specimen. This can be further expanded to include multiple specimens. This can also be extended to other tissue types.