This page provides specifications of knee specimen OKS003. This is a young, healthy, female specimen.

Specimen Characteristics

Left knee

Gender: Female

Age: 25 years

Race: White

Height: 1.73 m

Weight: 68 kg

BMI: 22.8

Serologically tested: Yes.

X-ray:

Experimentation Workflow

Experimentation

-- aerdemir 2014-11-30 23:52:27 All, please complete relevant sections of this page during and after experimentation.

Specimen

Reference Specification: Specifications/Specimens - Revision as of November 30, 2014

Leading Team Member: Snehal Chokhandre

Supporting Team Members: Ahmet Erdemir

Timeline: November 30, 2014 @ ~ 9:00 AM (specimen pulled out of freezer)

Data Location: In this page.

Protocol Deviations:

  1. None.

Notes:

  1. None.

Specimen Preparation

Reference Specification: Specifications/SpecimenPreparation - Revision as of November 30, 2014

Leading Team Member: Tara Bonner

Supporting Team Members: Snehal Chokhandre, Craig Bennetts, Ahmet Erdemir

Timeline:

Data Location:

Protocol Deviations:

  1. Femur registration markers were assembled medially, laterally, and posteriorly (rather than anteriorly or anteromedially). Registration marker probing order was also changed to lateral, medial, posterior. Tibia registration markers were assembled per the specification. Probing order is the same as the specification: lateral medial posterior.
  2. Ten points (not twelve) were collected on each registration marker. The specifications may need to change as per Robb this has been how the system was set up all the time.

Notes:

  1. Dissected and keeping skin-fat-muscle layers from thigh and shank.
  2. Patella Optotrak marker needed to be replaced. Probing of patella registration marker was repeated for this reason.

Joint Imaging

Specimen Preparation

Reference Specification: Specifications/SpecimenPreparation - Revision as of November 30, 2014

Leading Team Member: Craig Bennetts

Supporting Team Members: Snehal Chokhandre

Timeline: ~3:00 PM - 3:30 PM December 1, 2014 (patella registration marker will be placed next day)

Data Location:

Protocol Deviations:

  1. None.

Notes:

  1. None.

Imaging

Reference Specification: Specifications/ExperimentationAnatomicalImaging - Revision as of December 1, 2014

Leading Team Member: Craig Bennetts

Supporting Team Members: Snehal Chokhandre, Ahmet Erdemir

Timeline:

Data Location:

Data was received from the imaging facilities and uploaded to in-house data management server (http://cobicore.lerner.ccf.org/midas; only accessible within Cleveland Clinic network). Raw DICOM files were stored in the folder Open Knee(s) --> Private --> oks003 --> MRI --> DICOM

DICOM files were converted to NIfTI files, stored in the folder Open Knee(s) --> Private --> oks003 --> MRI --> NIFTI of the in-house data management server. NIfTI files were also disseminated to the public in the 'Downloads' section of the project website, https://simtk.org/home/openknee, under the package oks003.

Data storage and dissemination were accomplished based on the updated imaging specifications, see Revision as of April 16, 2015.

Protocol Deviations:

  1. None.

Notes:

  1. To be able to identify where the patella is after the specimen is in the opaque bag, a double sided tape was secured to connect the transportation fixture to the bag. This helps orienting the specimen better in the MRI.
  2. General purpose imaging

    general-purpose.png

  3. Cartilage imaging

    cartilage.png

  4. Connective tissue imaging

    connective-tissue.png

Joint Mechanics

Equipment Preparation

Reference Specification: Specifications/PressureCalibration - Revision as of December 2, 2014

Leading Team Member: Robb Colbrunn

Supporting Team Members: Ahmet Erdemir, Nicole Nassif

Timeline: ~1:30 PM - ~2:00 PM December 2, 2014

Data Location:

Protocol Deviations:

  1. None.

Notes:

  1. Sensor model is 5051, label is 5051-P1-16813DT1-1200.
  2. A single pass equilibration at 6 bars was conducted.
  3. A double pass equilibration was conducted at an additional pressure level of 3 bars, by using single pass 6 bars data. Software reordered the passes.

Specimen Preparation

Reference Specification: Specifications/SpecimenPreparation - Revision as of November 30, 2014

Leading Team Member: Tara Bonner

Supporting Team Members:

Timeline:

Data Location:

Protocol Deviations:

  1. None.

Notes:

  1. None.

Testing

Reference Specification: Specifications/ExperimentationJointMechanics - Revision as of December 3, 2014

Leading Team Member: Tara Bonner

Supporting Team Members: Robb Colbrunn

Timeline:

Data Location:

Data was received from the robotics testing facility and uploaded to in-house data management server (http://cobicore.lerner.ccf.org/midas; only accessible within Cleveland Clinic network). Zipped folders were stored in the folder Open Knee(s) --> Private --> oks003 --> JointMechanics under relevant joint related folders.

Zipped folders were extracted and organized in the dissemination folder under Open Knee(s) --> Private --> oks003 --> JointMechanics --> DISSEMINATION of the in-house data management server. DISSEMINATION folder was downloaded and disseminated to the public in the 'Downloads' section of the project website, https://simtk.org/home/openknee, under the package oks003.

Data storage and dissemination were accomplished based on the updated joint mechanics testing specifications, see Revision as of October 13, 2015.

Protocol Deviations:

  1. None.

Notes:

  1. In tibiofemoral joint testing, passive flexion (for coordinate system optimization) was conducted at 50 N compressive load. Specifications state a 100 N compressive load should be used. Nonetheless, as noted by Tara, all previous specimens has been tested under 50 N compressive load for coordinate system optimization. The relevant protocol may need to be updated this.
  2. In tibiofemoral joint testing, robotic testing of combined loading at 90 degrees flexion ended prematurely. An additional trial was conducted to complete the tests.
  3. In patellofemoral joint testing, a coordinate system transformation matrix in SimVitro (the matrix that establishes the relationship between the tibia and load cell) needed to be mirrored to accommodate the left knee.

  4. In patellofemoral joint testing, at 0 degrees flexion multiple trials were collected for 20, 100, 200, 300, 400, 500 N quadriceps loading. This was necessitated by troubleshooting tendon freeze clamping and tendon actuator response.
  5. Version of simVITRO software used was unreleased revision 3024 of the repository. The files can be found in svn://dev.lerner.ccf.org/data1/svn/repos/colbrunn-svn/Projects/Numbered Projects/2013CB-031 Open Knee/Original simVITRO-Tested on oks001 oks002 oks003 oks004

Tissue Mechanics

Specimen Preparation

Reference Specification:

Leading Team Member: Snehal Chokhandre

Supporting Team Members: Craig Bennetts

Timeline:

Data Location:

Protocol Deviations:

  1. Due to delay in patellofemoral joint testing, tissue dissection was not completed on the same day; all ligament, tendon, menisci segments were dissected and frozen. Bone and cartilage were kept in the fridge another night for dissection in the following morning.

Notes:

  1. Various secondary tissues were dissected and stored: semitendinosus, patellofemoral ligament (medial and lateral), lateral tibiofemoral capsule, fat bursa, transverse ligament.

Testing

Reference Specification:

Leading Team Member:

Supporting Team Members:

Timeline:

Data Location:

Protocol Deviations:

  1. None.

Notes:

  1. None.

Data Analysis

Joint mechanical testing

Pictured below are the Anterior Posterior laxity graph, the IR/ER laxity graph, and the Varus/Valgus laxity graph.

oks006_2LaxityGraphs.png

Tissue mechanical testing

Cartilage

Location

Test

Sample name

Data/results

Patella

Unconfined compression

Patella

Confined compression

Patella

Uniaxial tension

Medial femoral condyle

Unconfined compression

Medial femoral condyle

Confined compression

Medial femoral condyle

Uniaxial tension

Lateral femoral condyle

Unconfined compression

Lateral femoral condyle

Confined compression

Lateral femoral condyle

Uniaxial tension

Patellar groove

Unconfined compression

Patellar groove

Confined compression

Patellar groove

Uniaxial tension

Medial tibial plateau

Unconfined compression

Medial tibial plateau

Confined compression

Medial tibial plateau

Uniaxial tension

Medial tibial plateau

Unconfined compression

Medial tibial plateau

Confined compression

Medial tibial plateau

Uniaxial tension

Modeling & Simulation

oks003 (last edited 2019-06-19 16:55:44 by owings)