• Specifications
• SpecimenPreparation

Target Outcome

The goal of this protocol is to prepare specimen for all experimentation; specifically, MRI, CT, and Ultrasound.

Procedures

Specimen Storage

1. Specimen Storage
   1. Storage Location
      1. Specimens should be stored in the walk-in freezer on the third floor in room ND3-77B
      2. Serological testing sheets should be scanned and saved in the repository directory upon delivery
      3. A log should be filled out on the data sheet found in ND3-77B with:
         1. Specimen number
         2. PI name being Ahmet Erdemir
         3. Shelf where the leg was placed labeled accordingly
         4. Source: Human
      4. Serological testing sheets for each specimen should be accounted for and should remain in room ND3-77B so long as a specimen still remains in the freezer.
   2. Specimen Packaging
      1. Specimens can be directly placed in the freezer in their original packaging after being procured from the company
      2. Specimens should be packaged as followed after handling it:
         1. Wrap specimen in a blue chuck (found in cabinet in room ND1-04).
         2. Specimen chuck should be labeled with the specimen number, the side (right or left), and what it is (leg or arm)
         3. The wrapped specimen should be placed in a red biohazard bag found in the same cabinet in ND1-04 as the chucks and labeled in the same fashion as the chuck.

Specimen Thawing

• The specimen should be taken to the BioRobotics Cadaver Lab in room ND1-04 and thawed for greater than 24 hours prior to specimen preparation at room temperature.

• For thawing periods greater than 24 hours (i.e. through the weekend) leave the specimen wrapped.
• For thawing periods less than 24 hours unwrap specimen

Preparation for MRI/CT

• The specimen is double bagged using sterile techniques and placed in a duffle bag for transport to MRI and CT facilities.
• First, the spherical registration markers must be attached. Spherical markers are hollow plastic spheres (10 mm radius ) that are attached to the bones using nylon screws (#8-32) for MRI registration. The screw axis and sphere centers should be concentric.

• Registration marker locations

• 

• 

Arm

• 7 markers should be placed on the arm
  • 4 on humerus
  • 3 on radius

Leg

• 12 markers should be placed on the leg
  • 6 on femur
  • 6 on tibia
• Ensure specimen is thawed as per thawing guidelines
• Place specimen in fixture in correct anatomical position
• One location at a time, pre-drill a 0.136" hole, tap with an 8-32 tap, and screw in marker.
• Drill, tap, place marker in each location
• Be sure each marker is secure.