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Contents

  1. PART I: Indentation
    1. Logistics
  2. Target Outcome
  3. Prerequisites
    1. Specifications
    2. Infrastructure
  4. Protocols
    1. Input
    2. Preparation
      1. Supplies/Equipment needed for experimentation
      2. Specimen Preparation Imaging and Indentation
      3. Preparing Ultrasound
    3. Procedures
      1. Antropometric Measurements
      2. Optotrak Marker Assembly on Bones
      3. Acquisition of Registration Marker Locations and Anatomical Landmarks
        1. Registration marker data collection
        2. Anatomical Landmark data collection
      4. Ultrasound Indentation
      5. Tissue Harvesting
      6. Mechanical Testing
        1. Preparation
        2. Testing
    4. Output
  5. Preliminary Work

PART I: Indentation

See Specifications/InVitroTesting/PartII for Part II: Surgery

Logistics

Part 1 - Indentation

Day 1

Day 2

Day 3

Morning

Thaw Specimen

Anthropometric/MRI Prep 8-9:30

Harvest Tissue

Mid

Thaw Specimen

CT & MRI 10-12

Freeze

Afternoon

Thaw Specimen

Ultrasound

Target Outcome

  • Demographic documentation (age, gender)
  • Anthropometric measurements (body mass, height, extremity lengths and circumferences)
  • A set of MR and CT images of upper and lower leg.
  • Ultrasound images at unloaded state to extract tissue thickness for various extremity locations
  • Ultrasound imaging during indentation to extract tissue thickness for various extremity locations
  • Mechanical testing of tissue and tissue-interface to characterize mechanical properties at ultrasound indentation locations

Prerequisites

Specifications

Infrastructure

Protocols

Input

Cadaver upper and lower legs and arms of the same donor

Preparation

Supplies/Equipment needed for experimentation

  • Supplies: scalpel, forceps, 16 registration markers, Optotrak sensor (for specimen), Optotrak sensor (for ultrasound), 2 drills, drill bit+tap 8-32 (for registration markers), drill bit (pre-drill for screws), drill bit+tap (for Optotrak sensor plug), ultrasound gel, pens, washable markers, cloth tape measure, wash cloths
  • Equipment: Optotrak camera system, Optotrak probe, Ultrasound system with foot-switch, 14L5 probe, 9L4 probe, probe casings, load-transducer computer, load-transducer unit, ATI IFPS box, blue DAQ unit, beige DAQ unit, cadaver fixture

Specimen Preparation Imaging and Indentation

One specimen (e.g. Upper and Lower Leg) will be used at a time

Preparing Ultrasound

Refer to in vivo protocol

Procedures

Antropometric Measurements

Refer to in vivo protocol

Optotrak Marker Assembly on Bones

  • Mounting of Optotrak markers:

  • Leg

    1. Place the femur Optotrak marker on femur base plug
    2. Place the tibia Optotrak marker on tibia base plug

  • Arm

    1. Place the radius Optotrak marker on radius base plug
    2. Place the humerus Optotrak marker on humerus base plug

Acquisition of Registration Marker Locations and Anatomical Landmarks

Registration marker data collection

  • RegistrationOrder.png

  • ArmRegistrationOrder.png

    1. Keep specimen within Optotrak measurement view.
    2. Use the digitizing probe to record registration marker locations along with Optotrak marker position/orientation output, measured with respect to the global Optotrak coordinate system for each respective bone
    3. Ten points on each spherical marker should be digitized such that they are distributed evenly about the sphere surface
    4. The spheres should be digitized in the following order:

Leg

  1. Proximal Femur
    1. F1. Central
    2. F2. Lateral
    3. F3. Medial
  2. Distal Femur
    1. F4. Lateral epicondyle
    2. F5. Anterior
    3. F6. Medial epicondyle
  3. Proximal Tibia
    1. T1. Laterial tibial condyle
    2. T2. Tibial tuberosity
    3. T3. Medial tibial condyle
  4. Distal Tibia
    1. T4. Shaft
    2. T5. Distal anterior lateral surface
    3. T6. Medial malleolus

Arm

  1. Humerus
    1. H1. Anterior proximal head
    2. H2. Lateral proximal
    3. H3. Medial proximal
    4. H4. Later epicondyle
  2. Radius
    1. R1. Neck of radius
    2. R2. Lateral distal
    3. R3. Anterior lateral

Anatomical Landmark data collection

  1. Keep specimen within Optotrak measurement view.
  2. Use the digitizing probe to record anatomical landmark locations along with Optotrak marker position/orientation output, measured with respect to the global Optotrak coordinate system for each respective bone.
  3. The following anatomical landmarks will be collected:

Leg

  1. Femur
    1. Lateral Femoral Epicondyle
    2. Medial Femoral Epicondyle
    3. Femoral Head Point 1
    4. Femoral Head Point 2
    5. Femoral Head Point 3
    6. Femoral Head Point 4
  2. Tibia
    1. Lateral Tibial Plateau
    2. Medial Tibial Plateau
    3. Lateral Malleolus
    4. Lateral Malleolus (again)
    5. Medial Malleolus
    6. Medial Malleolus (again

Arm

  1. Humerus
    1. Lateral Epicondyle
    2. Medial Epicondyle
    3. Humeral Head Point
  2. Ulna
    1. Lateral Epicondyle
    2. Medial Epicondyle
    3. Ulnar Styloid

Note that the specifications dictate that all specimens should be from the right side. If the data collection software is used to collect data from a left specimen, the data file labels will be incorrect as right handed coordinate systems will be created for the left knee. The following chart describes the anatomical meaning for each segment.

Table-Right_Left Abstraction.jpg

Segment Coordinate system definition (Right and Left)

Left

Right

Left

Right

Ultrasound Indentation

Refer to in vivo wiki as it is the same protocol.

Tissue Harvesting

A region of tissue (skin fat muscle) will be excised from the indentation region of the thigh. This will be labeled and immediately frozen as is. Samples will be cut to size prior to mechanical testing.

Mechanical Testing

Need:

  • Skin and Muscle Punch (dumbbell)
  • Fat Punch (cylinder)

Preparation

Six equally sized samples will be collected from the indentation region. The region will be excised, then samples will be dissected.

  • Skin
    • 3 dumbbell strips at 45 degree orientation to each other using punch

  • Fat
    • 1 cylindrical sample
      • how will this be excised?

  • Muscle
    • 1 cylindrical sample along transverse axis
      • how will this be excised?
    • 1 dumbbell shaped sample

  • Equally sized samples will be collected from the various interfaces
    • Skin-fat
    • Skin-muscle
    • Muscle-muscle
    • Fat-muscle (if any)
  • 4 samples from each interface will be collected
    • Cylindrical shape across the interface
    • Strip across interface
    • Strip along interface (tensile test)
    • Strip along interface (shear test)

Testing

Tensile Testing

  • Samples to be tested
    • 3 dumbbell skin
    • 1 dumbbell muscle
    • Strip across interface (4 total)
    • Strip along interface (4 total)

Compression (unconfined)

  • Samples to be tested
    • Cylinder fat
    • Cylinder muscle ( + fascia) transverse axis
    • Cylinder samples across each interface (4 total)

Failure Tests

  • yield, ultimate strength, failure strain each tissue sample

Output

Execution of specifications. see InVitroExperiments

Preliminary Work

Specifications/InVitroTesting (last edited 2019-03-06 19:24:16 by owings)